I am completely novice to miRNA field. Just started reading and getting information. Can you expert please answer my questions?
- How do you make sure that miRNA exists in your treatment samples? For example, you isolate miRNA using the kits available in the market. How will you identify which miRNA is that ? If it is first of its kind?
- I read about qPCR for miRNA but there also you need forward primer which should be specific to your miRNA, how will you do thatn when you don't know which miRNA is that.
There are so many questions, can somebody share some links where the basic questions are answered?
Please experts help me!
Edited by neuron, 28 January 2016 - 04:11 AM.