2 replies to this topic

[wswr]

In all the dilution plating protocols I find online, they said to incubate the plates with diluted samples for 24 - 48 hours. Why so long? When I plate a culture, I get single colonies in 12 - 16 hours. Is there a biochemical reason for such a long incubation time? Can I just do 12 - 16 hours?

[Michael Starr]

Wouldn't your plates overgrow and your colonies mix if you incubated them 24-48 hours? Do you really have any option?

 

It probably depends on the growth rate of the strain used.

[Andrea Fortina]

This time depends on the time strain needed to grow. Protocol could be use for most situation, but  you may adjust according to the specific circumstances.