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Dilution plating incubation time


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#1 wswr

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Posted 20 January 2016 - 09:16 AM

In all the dilution plating protocols I find online, they said to incubate the plates with diluted samples for 24 - 48 hours. Why so long? When I plate a culture, I get single colonies in 12 - 16 hours. Is there a biochemical reason for such a long incubation time? Can I just do 12 - 16 hours?



#2 Michael Starr

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Posted 27 January 2016 - 07:31 PM

Wouldn't your plates overgrow and your colonies mix if you incubated them 24-48 hours? Do you really have any option?

 

It probably depends on the growth rate of the strain used.



#3 Andrea Fortina

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Posted 15 June 2016 - 08:03 PM

This time depends on the time strain needed to grow. Protocol could be use for most situation, but  you may adjust according to the specific circumstances.


Andrea Fortina

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CreativeBiomart, Recombinant protein expert

 

 





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