Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

fragmentation single cells

fragmentation

  • Please log in to reply
No replies to this topic

#1 molbio1234

molbio1234

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 61 posts
2
Neutral

Posted 18 January 2016 - 04:26 AM

Hello,

I really need some advice. I am sorting bulk cell population into single cells, then lysing the single cell. I need to next fragment the single cell in preparation for NGS (end repair, a tailing). Can anyone recommend a fragmentation method? I can't use Covaris as it is a single cell only. I need to use an enzymatic shearing but don't know what will work.

 

Has anyone any experience? thanks.






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.