Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

What microscope magnification to use to count fibroblasts and lymphoblasts

cell culture lymphoblasts cell counting

  • Please log in to reply
1 reply to this topic

#1 Irina Duff

Irina Duff

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 10 January 2016 - 04:43 PM

Hello Everyone!

 

I have a really burning question. I have just started working with cell culture.

When I count cells on 10x magnification, I have one quantity of cells, when I look at cells on 200x magnification, I see more cells, which is obvious. I would really appreciate the explanation: what magnification should I use to count cells?

 

Thanks in advance



#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,233 posts
486
Excellent

Posted 11 January 2016 - 01:39 AM

I'm assuming here that you are using a hemocytometer to do this?

 

You should use a 10x objective - 100x magnification, such that one of the large squares bounded by 3 lines takes up most of the field of view. 200x objectives would be rare and far too high a magnification to count cells (you would see 1-5 in a field).







Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.