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What microscope magnification to use to count fibroblasts and lymphoblasts

cell culture lymphoblasts cell counting

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#1 Irina Duff

Irina Duff


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Posted 10 January 2016 - 04:43 PM

Hello Everyone!


I have a really burning question. I have just started working with cell culture.

When I count cells on 10x magnification, I have one quantity of cells, when I look at cells on 200x magnification, I see more cells, which is obvious. I would really appreciate the explanation: what magnification should I use to count cells?


Thanks in advance

#2 bob1


    Thelymitra pulchella

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Posted 11 January 2016 - 01:39 AM

I'm assuming here that you are using a hemocytometer to do this?


You should use a 10x objective - 100x magnification, such that one of the large squares bounded by 3 lines takes up most of the field of view. 200x objectives would be rare and far too high a magnification to count cells (you would see 1-5 in a field).

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