I run recently two 12-well precast gels (Bio-rad) at the same time. I loaded 20 ul of sample in each lane (max. allowed) and I did it relatively slowly in order to avoid getting sample out of the wells while loading.
When I blotted for Actin (45 kDa) the next day, I noticed that the bands from individuals lanes were sort of connected with each other by small, curved lines like ears. I did not get the same pattern for histones (low MW) though.
Could the observed pattern on the actin blot mean a spillover between the lanes? I have attached an image showing the blot.
Thank you very much in advance,