My team and I are working to set up assays in which we would like to use oligodendrocytes isolated from brain material. We're collaborating with a lab in London (we're in the Netherlands) and the plan is to have the lab in London perform the in vivo experiment, which is their expertise, and then have them send us whole brains obtained from the experimental rats. However, as we would like to isolate the oligodendrocytes from these brains in our lab in the Netherlands, a method for proper transport is required so that the tissue remains vital during the transport. In vitro experimentation is our expertise, so splitting the work in this way would be most efficient.
I was wondering whether anyone has any experience with this? Do you suspend the brain in its entirety in a certain buffer, do you need to perfuse them with a specific buffer prior to isolation from the animals, or is it perhaps better to isolate the oligodendrocytes in London and transport only these cells?
I would love to hear your input on this!