I want to measure a total protein amount of an insect (beetle or ant)
My question is how do i separate (extract) the proteins and then quantify them:
- what lysis buffer should i use for it if i want to measure it with bradford method?
and what do you think is the best method to measure the total amount of proteins? bradford, PCA, or something else?
thanks a lot and i would appreciate any answer
Have a great day