First, let me say that I agree with the general idea that routine use of antibiotics in cell culture is not recommended. However, in the lab where I work we routinely use 1% antibiotic/antimycotic in media.
Second, I have come across information recommending reducing antibiotic/antimycotic concentration by at least 50% if Serum-Free Media is being used (again, ideally none at all).
Lately we have been trying to differentiate some cells, which calls for the reduction of serum in culture from 10% to 1%. My question is, how much should I reduce my antibiotic/antimycotic concentration if I have to use it? I was originally thinking at least 50% reduction.
Thanks for all your help!
Edited by yl2j, 30 December 2015 - 08:53 PM.