I loaded a protein extract on glycerol gradient (10-50%) and spun at 55000 rpm for 2 hrs. I could see my protein of interest running in 5th fraction. I want to know the molecular weight of that protein mixture. Any Idea how to do that?
After asking Dr. google, I found out that we need to calculate sedimentation coefficient using known protein and then convert that into the molecular weight for unknown protein. Still I am not able to figure that out
If anybody has done these kind of experiment. Please help me with your experience.