Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

ELISA antibody's

ELISA antibody coatingbuffer

Best Answer bob1, 09 December 2015 - 12:07 PM

Probably not -it  is pretty routine for ELISAs to have a step like this. Elution at high pH is usually done at pH 10.5 or above in the presence of low salt.

Go to the full post


  • Please log in to reply
2 replies to this topic

#1 Levi Ooms

Levi Ooms

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 09 December 2015 - 11:05 AM

Hi,

I am doing an ELISA. now the protocol says to dissolve the primair antibody into Coatingbuffer(pH 9.4). isn't this a problem for the antibody with relations to the chance it will denature because of the high pH value?

 

Greets,

Levi



#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,512 posts
543
Excellent

Posted 09 December 2015 - 12:07 PM   Best Answer

Probably not -it  is pretty routine for ELISAs to have a step like this. Elution at high pH is usually done at pH 10.5 or above in the presence of low salt.



#3 Zoe Winslet

Zoe Winslet

    member

  • Active Members
  • Pip
  • 9 posts
0
Neutral

Posted 15 June 2016 - 06:52 PM

there is no problem with the protocol


Be best yourself!






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.