2 replies to this topic

[hilarync]

Has anyone tried incubating two membranes in the same container when using the same primary?  I figure it might be possible to use a little more solution than I would normally use for one membrane, but still save a bunch of antibody compared to two separate containers. 

Thanks!  Hilary

[bob1]

Yes, and it works, but you just need to ensure that you have enough solution that both membranes get access to the solution. This can be achieved by having a bigger volume that you might use for 1 membrane or by switching the positions of the membranes (top to bottom) halfway through the incubation (and doing a longer incubation). You could also do them sequentially.

[hilarync]

Thank you!  I will give it a go.