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Can I incubate two membranes in one container?

Western blotting

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#1 hilarync

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Posted 02 December 2015 - 07:03 AM

Has anyone tried incubating two membranes in the same container when using the same primary?  I figure it might be possible to use a little more solution than I would normally use for one membrane, but still save a bunch of antibody compared to two separate containers. 

Thanks!  Hilary



#2 bob1

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Posted 02 December 2015 - 11:19 AM

Yes, and it works, but you just need to ensure that you have enough solution that both membranes get access to the solution. This can be achieved by having a bigger volume that you might use for 1 membrane or by switching the positions of the membranes (top to bottom) halfway through the incubation (and doing a longer incubation). You could also do them sequentially.



#3 hilarync

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Posted 02 December 2015 - 03:05 PM

Thank you!  I will give it a go.







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