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PROTOCOL FOR LINEARIZED DNA


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#1 pana

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Posted 26 November 2015 - 04:10 AM

   I want to linearize my plasmid so i will be able to transfect it 

The enzyme that i will use is Notl 

 

anyone have experience with such protocol?

 

10X



#2 bob1

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Posted 26 November 2015 - 01:27 PM

Basically mix the DNA with the appropriate restriction buffer and enzyme in a final volume of 50 ul. Digest for 1 hour at 37. Check digest has worked by running on gel. Purify DNA and use for transfection.






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