Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Different Accession numbers

NCBI nucleotide sequence

  • Please log in to reply
3 replies to this topic

#1 Mad Researcher

Mad Researcher

    Newbie !!

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 205 posts
12
Good

Posted 25 November 2015 - 02:48 AM

Hi,

 

I am searching for a gene but I find different accession numbers associated with it. For example: If I search for TLR8 in humans I have 5 different accession numbers:

 

NG_012882 - 

Homo sapiens toll-like receptor 8 (TLR8), RefSeqGene on chromosome X

NM_016610 - 

Homo sapiens toll-like receptor 8 (TLR8), transcript variant 1, mRNA

NM_138636 - 

Homo sapiens toll-like receptor 8 (TLR8), transcript variant 2, mRNA

XM_011545529 - 

PREDICTED: Homo sapiens toll-like receptor 8 (TLR8), transcript variant X1, mRNA

XM_011545530 - 

PREDICTED: Homo sapiens toll-like receptor 8 (TLR8), transcript variant X2, mRNA

My question is why are there 5 different sequences, what does each represent and which one is the correct one to chose? 

What are predicted genes?

 

Thanks


Cheers,

Mad Researcher

#2 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 3,115 posts
175
Excellent

Posted 25 November 2015 - 04:51 AM

just based on the descriptions, i would take the first one (refseqgene). it appears to be the rference sequence. the other four are variants or possible variants of the wild-type gene.


talent does what it can
genius does what it must
i do what i get paid to do

#3 phage434

phage434

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 2,733 posts
298
Excellent

Posted 25 November 2015 - 05:31 AM

The transcript entries are also likely spliced, while the refseq would not be.



#4 Mad Researcher

Mad Researcher

    Newbie !!

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 205 posts
12
Good

Posted 25 November 2015 - 09:09 AM

The transcript entries are also likely spliced, while the refseq would not be.

 

And what about the predicted ones?


Cheers,

Mad Researcher





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.