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# Serial dilution

serial dilution

Best Answer mdfenko, 20 November 2015 - 04:46 AM

take part of the 1ng/ml and add an equal volume of diluent to make 0.5ng/ml.

take part of the 0.5ng/ml and add an equal volume of diluent to make 0.25ng/ml.

take part of the 0.25ng/ml and add an equal volume of diluent to make 0.125ng/ml.

etc.

So, 1 ng/ml is my stock and if it comes in 1ml then:
I take 0.5 ml of my stock + 0.5 of H2O -- 1

0.5 ml of 1 + 0.5 ml of H2O -- 2

0.5 ml of 2 + 0.5 ml of H20 -- 3

and so on

Am i correct?

if 1, 2 and 3 are 0.5ng/ml, 0.25ng/ml and 0.125ng/ml, respectively, then yes.

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6 replies to this topic

### #1 Mad Researcher

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Posted 18 November 2015 - 11:42 PM

Hello,

I am confused on how to do a serial dilution.

It says:

Dilute standard from 1ng/ml to zero in diluent.

And I want to have 5 or 6 points:

1. 1ng/mL

2. 0.5ng/ml,

3. 0.25 ng/ml,

4. 0.125 ng/ml (125pg/ml),

5. 62.5 pg/ml,

6. 31.25 pg/ml

Kindly advice how can I serially dilute this.

Cheers,

### #2 pito

pito

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Excellent

Posted 19 November 2015 - 03:25 AM

Hello,

I am confused on how to do a serial dilution.

It says:

Dilute standard from 1ng/ml to zero in diluent.

And I want to have 5 or 6 points:

1. 1ng/mL

2. 0.5ng/ml,

3. 0.25 ng/ml,

4. 0.125 ng/ml (125pg/ml),

5. 62.5 pg/ml,

6. 31.25 pg/ml

Kindly advice how can I serially dilute this.

you want to go from 1ng/ml to 31,25pg/ml ?

If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.

### #3 mdfenko

mdfenko

an elder emeritus

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• 3,265 posts
198
Excellent

Posted 19 November 2015 - 06:07 AM

take part of the 1ng/ml and add an equal volume of diluent to make 0.5ng/ml.

take part of the 0.5ng/ml and add an equal volume of diluent to make 0.25ng/ml.

take part of the 0.25ng/ml and add an equal volume of diluent to make 0.125ng/ml.

etc.

talent does what it can
genius does what it must
i do what i get paid to do

### #4 Mad Researcher

Newbie !!

• Active Members
• 246 posts
13
Good

Posted 19 November 2015 - 06:13 AM

Hello,

I am confused on how to do a serial dilution.

It says:

Dilute standard from 1ng/ml to zero in diluent.

And I want to have 5 or 6 points:

1. 1ng/mL

2. 0.5ng/ml,

3. 0.25 ng/ml,

4. 0.125 ng/ml (125pg/ml),

5. 62.5 pg/ml,

6. 31.25 pg/ml

Kindly advice how can I serially dilute this.

you want to go from 1ng/ml to 31,25pg/ml ?

Yes...

Cheers,

### #5 Mad Researcher

Newbie !!

• Active Members
• 246 posts
13
Good

Posted 19 November 2015 - 06:15 AM

take part of the 1ng/ml and add an equal volume of diluent to make 0.5ng/ml.

take part of the 0.5ng/ml and add an equal volume of diluent to make 0.25ng/ml.

take part of the 0.25ng/ml and add an equal volume of diluent to make 0.125ng/ml.

etc.

So, 1 ng/ml is my stock and if it comes in 1ml then:
I take 0.5 ml of my stock + 0.5 of H2O -- 1

0.5 ml of 1 + 0.5 ml of H2O -- 2

0.5 ml of 2 + 0.5 ml of H20 -- 3

and so on

Am i correct?

Cheers,

### #6 pito

pito

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• Global Moderators
• 1,464 posts
100
Excellent

Posted 19 November 2015 - 12:33 PM

take part of the 1ng/ml and add an equal volume of diluent to make 0.5ng/ml.

take part of the 0.5ng/ml and add an equal volume of diluent to make 0.25ng/ml.

take part of the 0.25ng/ml and add an equal volume of diluent to make 0.125ng/ml.

etc.

So, 1 ng/ml is my stock and if it comes in 1ml then:
I take 0.5 ml of my stock + 0.5 of H2O -- 1

0.5 ml of 1 + 0.5 ml of H2O -- 2

0.5 ml of 2 + 0.5 ml of H20 -- 3

and so on

Am i correct?

if your stock is 1ng/ml and you then take 0,5ml of this and add 0,5ml water you will have in total 0.5ng (since you took 0.5ml from a 1ng/1ml stock) of the stock in 1ml, thus 0.5ng/ml.

And you can keep doing this:

take 0,5ml from the "new" stock , add 0.5ml water... you keep diluting it 1/2 each step.

But this is pretty basic, you should understand this, know how this works.

If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.

### #7 mdfenko

mdfenko

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• 3,265 posts
198
Excellent

Posted 20 November 2015 - 04:46 AM   Best Answer

take part of the 1ng/ml and add an equal volume of diluent to make 0.5ng/ml.

take part of the 0.5ng/ml and add an equal volume of diluent to make 0.25ng/ml.

take part of the 0.25ng/ml and add an equal volume of diluent to make 0.125ng/ml.

etc.

So, 1 ng/ml is my stock and if it comes in 1ml then:
I take 0.5 ml of my stock + 0.5 of H2O -- 1

0.5 ml of 1 + 0.5 ml of H2O -- 2

0.5 ml of 2 + 0.5 ml of H20 -- 3

and so on

Am i correct?

if 1, 2 and 3 are 0.5ng/ml, 0.25ng/ml and 0.125ng/ml, respectively, then yes.

talent does what it can
genius does what it must
i do what i get paid to do