Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Serial dilution

serial dilution

Best Answer mdfenko, 20 November 2015 - 04:46 AM

 

take part of the 1ng/ml and add an equal volume of diluent to make 0.5ng/ml.

 

take part of the 0.5ng/ml and add an equal volume of diluent to make 0.25ng/ml.

 

take part of the 0.25ng/ml and add an equal volume of diluent to make 0.125ng/ml.

 

etc.

So, 1 ng/ml is my stock and if it comes in 1ml then: 
I take 0.5 ml of my stock + 0.5 of H2O -- 1

0.5 ml of 1 + 0.5 ml of H2O -- 2

0.5 ml of 2 + 0.5 ml of H20 -- 3

 

and so on

 

Am i correct?

 

if 1, 2 and 3 are 0.5ng/ml, 0.25ng/ml and 0.125ng/ml, respectively, then yes.

Go to the full post


  • Please log in to reply
6 replies to this topic

#1 Mad Researcher

Mad Researcher

    Newbie !!

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 205 posts
12
Good

Posted 18 November 2015 - 11:42 PM

Hello,

 

I am confused on how to do a serial dilution. 

 

It says:

Dilute standard from 1ng/ml to zero in diluent.

And I want to have 5 or 6 points: 

1. 1ng/mL

2. 0.5ng/ml,

3. 0.25 ng/ml,

4. 0.125 ng/ml (125pg/ml),

5. 62.5 pg/ml,

6. 31.25 pg/ml 

 

Kindly advice how can I serially dilute this. 


Cheers,

Mad Researcher

#2 pito

pito

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,456 posts
100
Excellent

Posted 19 November 2015 - 03:25 AM

Hello,

 

I am confused on how to do a serial dilution. 

 

It says:

Dilute standard from 1ng/ml to zero in diluent.

And I want to have 5 or 6 points: 

1. 1ng/mL

2. 0.5ng/ml,

3. 0.25 ng/ml,

4. 0.125 ng/ml (125pg/ml),

5. 62.5 pg/ml,

6. 31.25 pg/ml 

 

Kindly advice how can I serially dilute this. 

 

you want to go from 1ng/ml to 31,25pg/ml ?


If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.


#3 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 3,160 posts
189
Excellent

Posted 19 November 2015 - 06:07 AM

take part of the 1ng/ml and add an equal volume of diluent to make 0.5ng/ml.

 

take part of the 0.5ng/ml and add an equal volume of diluent to make 0.25ng/ml.

 

take part of the 0.25ng/ml and add an equal volume of diluent to make 0.125ng/ml.

 

etc.


talent does what it can
genius does what it must
i do what i get paid to do

#4 Mad Researcher

Mad Researcher

    Newbie !!

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 205 posts
12
Good

Posted 19 November 2015 - 06:13 AM

 

Hello,

 

I am confused on how to do a serial dilution. 

 

It says:

Dilute standard from 1ng/ml to zero in diluent.

And I want to have 5 or 6 points: 

1. 1ng/mL

2. 0.5ng/ml,

3. 0.25 ng/ml,

4. 0.125 ng/ml (125pg/ml),

5. 62.5 pg/ml,

6. 31.25 pg/ml 

 

Kindly advice how can I serially dilute this. 

 

you want to go from 1ng/ml to 31,25pg/ml ?

 

Yes...


Cheers,

Mad Researcher

#5 Mad Researcher

Mad Researcher

    Newbie !!

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 205 posts
12
Good

Posted 19 November 2015 - 06:15 AM

take part of the 1ng/ml and add an equal volume of diluent to make 0.5ng/ml.

 

take part of the 0.5ng/ml and add an equal volume of diluent to make 0.25ng/ml.

 

take part of the 0.25ng/ml and add an equal volume of diluent to make 0.125ng/ml.

 

etc.

So, 1 ng/ml is my stock and if it comes in 1ml then: 
I take 0.5 ml of my stock + 0.5 of H2O -- 1

0.5 ml of 1 + 0.5 ml of H2O -- 2

0.5 ml of 2 + 0.5 ml of H20 -- 3

 

and so on

 

Am i correct?


Cheers,

Mad Researcher

#6 pito

pito

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,456 posts
100
Excellent

Posted 19 November 2015 - 12:33 PM

 

take part of the 1ng/ml and add an equal volume of diluent to make 0.5ng/ml.

 

take part of the 0.5ng/ml and add an equal volume of diluent to make 0.25ng/ml.

 

take part of the 0.25ng/ml and add an equal volume of diluent to make 0.125ng/ml.

 

etc.

So, 1 ng/ml is my stock and if it comes in 1ml then: 
I take 0.5 ml of my stock + 0.5 of H2O -- 1

0.5 ml of 1 + 0.5 ml of H2O -- 2

0.5 ml of 2 + 0.5 ml of H20 -- 3

 

and so on

 

Am i correct?

 

 

if your stock is 1ng/ml and you then take 0,5ml of this and add 0,5ml water you will have in total 0.5ng (since you took 0.5ml from a 1ng/1ml stock) of the stock in 1ml, thus 0.5ng/ml.

 

And you can keep doing this:

take 0,5ml from the "new" stock , add 0.5ml water... you keep diluting it 1/2 each step.

 

But this is pretty basic, you should understand this, know how this works.


If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.


#7 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 3,160 posts
189
Excellent

Posted 20 November 2015 - 04:46 AM   Best Answer

 

take part of the 1ng/ml and add an equal volume of diluent to make 0.5ng/ml.

 

take part of the 0.5ng/ml and add an equal volume of diluent to make 0.25ng/ml.

 

take part of the 0.25ng/ml and add an equal volume of diluent to make 0.125ng/ml.

 

etc.

So, 1 ng/ml is my stock and if it comes in 1ml then: 
I take 0.5 ml of my stock + 0.5 of H2O -- 1

0.5 ml of 1 + 0.5 ml of H2O -- 2

0.5 ml of 2 + 0.5 ml of H20 -- 3

 

and so on

 

Am i correct?

 

if 1, 2 and 3 are 0.5ng/ml, 0.25ng/ml and 0.125ng/ml, respectively, then yes.


talent does what it can
genius does what it must
i do what i get paid to do





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.