"A basal inorganic medium (pH 8.0–8.2) for enrichment and isolation of ammonium oxidation bacteria (AOB) was prepared by dissolving 0.5 g ammonium sulfate, [(NH4)2SO4 as a source of reduced N to investigate the acceptability of inorganic N sources for heterotrophic nitrification; 0.7 g KH2PO4, 0.5 g MgSO47H2O, 0.5 g CaCl22H2O and 1 mL of trace mineral solution in 1 L of distilled water. The trace mineral solution contained (per liter): 2.86 g H3BO3, 0.22 g ZnSO47H2O, 0.08 g CuSO4 5H2O, 2.03 g MnSO44H2O, and 1.26 g Na2MoO42H2O (pH 7.0). The AOB medium was autoclaved for 30 min at 121 C."
- X.P. Yang
Help, how do I prepare the stock solution from the chemicals? Do I mix them all in one step or should I have a way of mixing them? I'm having difficulties finding protocols for stock solution creation for ammonia-oxidizing bacteria medium, as all that they show are recipes and not how i should create them. thank you so much