I'm attempting to generate a humoral response against an antigen and will be using a terminal-cysteine conjugated peptide conjugated to KLH. I'm considering using Thermo's imject KLH maleimde for this purpose. I have a few questions regarding this:
Is the gel filtration/dialysis/desalting step really needed to remove EDTA? EDTA is generally tolerable at small doses in mice (the host I'll be using).
How do I verify/quantify conjugation efficiency?