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Diluting Inhibitors with DMSO for a WST-1 Assay

wst-1 assay cell culture DMSO dilutions

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#1 musicmaniac0428

musicmaniac0428

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Posted 06 November 2015 - 02:04 PM

So I'm looking to dilute my inhibitor from a stock solution of 5mM into the following concentrations for a WST-1 Assay run in triplicate 0.2μM, 0.39μM, 0.78μM, 1.56μM, 3.13μM and 6.25μM. I'll be adding 1μL of each inhibitor with 99μL of media to the wells and therefore would need to make up dilutions of 20μM, 39μM, 78μM, 156μM, 313μM and 625μM. I’ll be diluting the inhibitor with DMSO, however I need to maintain DMSO concentration at 1% in each of the wells. I will be treating the cells every 24 hours for 3 days and therefore need at least 9μL of each dilution so I want to make 20μL of each. I'm not entirely sure how to do this so I was hoping someone could walk me through the process/calculations to perform a serial dilution on this inhibitor while maintaining DMSO at 1% in each well. 







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