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Importance of certified pyrogen-free tubes?

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#1 seanspotatobusiness



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Posted 06 November 2015 - 07:08 AM

Any idea how much pyrogen is likely to in microcentrifuge tubes that aren't certified pyrogen-free? I want them to store plasmid for transfection.

#2 Trof


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Posted 07 November 2015 - 10:43 AM

For transfection of what? If you don't use it subsequently in humans or animals, you will hardly ever notice any pyrogen. These are small chemical substances, that are know to cause fever (i.e. activate the immune system) in injected into blood or so. If you use it for bacteria or cell culture, you don't have any immune system there.


For human or animal use, all things must be (I hope) pyrogen-free certified, so that there is no possible harm comming from a plastics. But otherwise it's just a "clean made" tube, that may have traces of whatever and you are only interested in those, that may damage your product, in case od plasmids it should be DNase-free.

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