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Protein Clevage

ATM

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#1 Prake

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Posted 01 November 2015 - 09:00 PM

Hello Everyone,

 

I am working on ATM (ataxia -telangiectasia mutant) protein, i found that the phosphorylated form of the protein presents two distinct bands, which i speculate that could be due to clevage by Caspase activation. What could be the other possible reasons for this

 

Thanks in advance and Appreciate the interesting suggestions. 



#2 mdfenko

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Posted 02 November 2015 - 05:44 AM

could be differential phosphorylation

 

or

 

some didn't phosphorylate (was one of the bands equal in migration with the unphosphorylated?)

 

which gel chemistry did you use to look at the effect(s) of phosphorylation?


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