I was reading about the use of 3-AT in the yeast two hybrid system. They use it to lower false positive as 3-AT is a competitive inhibitor of the his3 gene product.
But what if you would use another product that is a competitive inhibitor of another protein in the histidine pathway?
Or the other way around: if you use another reporter gene in that pathway and still use the 3-AT ?
Would this still be possible or?
I saw a paper where they used another reporter gene (his1) and they did not use 3-AT, which made me wonder about it