Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

yeast two hybrid 3-AT

  • Please log in to reply
No replies to this topic

#1 lyok



  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 209 posts

Posted 31 October 2015 - 08:34 AM

Hey all,

I was reading about the use of  3-AT in the yeast two hybrid system. They use it to lower false positive as 3-AT is a competitive inhibitor of the his3 gene product.

But what if you would use another product that is a competitive inhibitor of another protein in the histidine pathway?
Or the other way around: if you use another reporter gene in that pathway and still use the 3-AT ?

Would this still be possible or?

I saw a paper where they used another reporter gene (his1) and they did not use 3-AT, which made me wonder about it

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.