i am stucking in making SFM ( Soy bean Medium ) +MgCl2 agar.
How about using soy bean flour instead of Soy flour?
When ever i put the bottle that included SFM medium agar in Autoclave machine, in 121 degree celcius, after finishing, all media went out, and soy bean is precipitated.
I was tried to stir soy bean flour by magnetic bar for 5 hrs, then autoclave, but the result is same.
What should i do?
i really need your help, and appreciate any your help
Thank in advance