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Making SFM+MgCl2 agar for Conjugation

molecular cloning conjugation E.coli streptomyces media

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#1 Huongnguyen.miss

Huongnguyen.miss

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Posted 28 October 2015 - 02:06 AM

i am stucking in making SFM ( Soy bean Medium ) +MgCl2 agar. 

How about using soy bean flour instead of Soy flour?

When ever i put the bottle that included SFM medium agar in Autoclave machine, in 121 degree celcius, after finishing, all media went out, and soy bean is precipitated.

I was tried to stir soy bean flour by magnetic bar for 5 hrs, then autoclave, but the result is same.

What should i do?

i really need your help, and  appreciate any your help

Thank in advance 







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