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How to clean up PCR place & pipettes?

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#1 moljul



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Posted 24 October 2015 - 12:40 AM

Do anyone of you know, which chemical you can use to clean your bench to make it free of nucleic acids as well as nucleases? My boss don´t want to purchase special sprays or something!! It must be something, every lab has anyway - like hypochlorit or something like that!


Please let me know, how you clean your PCR place smile.png




#2 bob1


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Posted 24 October 2015 - 12:56 AM

This might help: http://journals.plos...al.pone.0013042


Basic solutions (e.g. hypochlorite or hydroxides) are pretty effective at removing ordinary DNA contamination levels. Pretty nothing works well to remove short DNA fragments (100 bp or so). The best bet there is to use the dUT-UNG system as most of these size fragments are generated by PCR.

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