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storage conditions for purified protein

stored temperature purified protein

Best Answer bob1, 19 October 2015 - 11:33 AM

It depends on the protein and what form it is in. Lyophilized protein can be stored in the fridge for a while, but lasts almost indefinitely in the freezer. Proteins in buffer will last a while in the freezer (dependent on freeze/thaw), and can last forever at -80, but again this is dependent on freeze/thaw cycles.

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#1 Meg P. Anula

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Posted 19 October 2015 - 04:36 AM

Hi all,

 

Can anyone enlighten me on the ideal storing temperature for purified protein?

 

And how long can it last (based on your experience) in said temperature?

 

ps: my application for the purified protein is to use on western blotting only. 

 

Thanks.



#2 bob1

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Posted 19 October 2015 - 11:33 AM   Best Answer

It depends on the protein and what form it is in. Lyophilized protein can be stored in the fridge for a while, but lasts almost indefinitely in the freezer. Proteins in buffer will last a while in the freezer (dependent on freeze/thaw), and can last forever at -80, but again this is dependent on freeze/thaw cycles.



#3 phage434

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Posted 19 October 2015 - 02:06 PM

Purified protein (such as restriction enzymes) are often stored at -20 in 50% glycerol to prevent freezing, which eliminates freeze/thaw issues.







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