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Illumina MiSeq

OTUs QIIME Microbiota

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#1 vanu



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Posted 14 October 2015 - 09:51 AM

Hi all,


I have recently received results from a microbiota analysis conducted by 300bp paired-end read (Illumina MiSeq) on faecal samples.


The information delivered contains lot of data such as 16S pre-processing and OTU picking, OTU diversity analyses with QIIME and so on. I am not very familiar with these kind of data since I have usually worked with relative abundances of taxa from 454 pyrosequencing results.


 So, I would really much appreciate your help with the following question:

In order to conduct statistical analyses, do I have to take into consideration  the OTU count table (OTUs count for each of the analysed samples)  or do I also have to take into account other information provided such as the OTU consensus taxonomy summary, sequence taxonomy summary...?


Thank you very much in advance for your help,


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