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Calculate DNA amount

calculation DNA concentration

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#1 p.blaze53

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Posted 29 September 2015 - 11:30 PM

I’d like prepare a reaction mix (total volume: 120 uL) containing 250 ng genomic DNA. The DNA concentration of my sample is 51 ng/uL in 65 uL RNAse free water. Does it mean I have to add  4.9 uL from my sample to the reaction?

 

51 ng DNA -> 1 uL

250 ng DNA -> ? uL

250/51 = 4.9 uL

 

Is my calculation correct? 



#2 bob1

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Posted 29 September 2015 - 11:40 PM

Yes - a simple way to check is to ask how many 50's there are in 250 - there are 5... 51 is slightly more than 50 so there will be slightly fewer 51's in 250...







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