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Using qPCR machine for simple PCR, is it possible?

RT PCR qPCR PCR

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3 replies to this topic

#1 p.blaze53

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Posted 29 September 2015 - 12:22 AM

Hi everyone! We've got only a qPCR machine in our lab, but I need to do a simple PCR. Maybe it is a stupid question, but I haven't got a clean answer yet.



#2 phage434

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Posted 29 September 2015 - 04:06 AM

Yes, you can simply ignore the fluorescence readings. But they are useful in monitoring a normal PCR, since you can tell how many cycles are really required to amplify your sample.



#3 p.blaze53

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Posted 29 September 2015 - 10:06 PM

Thank you for your useful answer!



#4 bob1

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Posted 30 September 2015 - 12:38 PM

You can also take the products of a qPCR (or regular PCR run on a qPCR machine) and run them on a gel like normal.







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