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Agarose Gel consistency issues

agarose gel electrophoresis

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#1 Barcoder

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Posted 28 September 2015 - 01:38 PM

So I recently began work in a different lab that hasn't been used in about two years, and I have yet to make a gel that hasn't completely fallen apart. 

I have made multiple 0.7%, 1%, and 1.5% gels, and obviously I expected the 0.7% gels to be fragile but even the 1.5% gels will just turn to mush if I try to move them from the mould. 

All gels were made with agarose from the same bottle, and 0.5X TBE from the same stock solution. Has anyone encountered this before or have any preventative tips?



#2 phage434

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Posted 28 September 2015 - 01:56 PM

A 1.5% gel should be very stable, almost rubbery. Your agarose is probably bad.  Another possibility is that the pH of the buffer you are using is way off.  An acidic pH can lead to fragile gels.



#3 Barcoder

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Posted 28 September 2015 - 02:06 PM

Thank you, I'll check the pH and then see if I can try some different agarose







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