I am using MDCK cells, and early this week I cultured cells in a very low density as we had a holiday for 3 days, and I forgot to to come during the holiday to change the medium for my cells.
when I come back, the medium was little acidic (but not so bad, still orange,), but cells looks not so good, with some air vacuoles or shiny points inside.
I cannot decide if my cells are bad or not, can u help me and give me ur comment.
I wonder if my cells exposed to a lot of stress/starvation and begin to die
Please check attached pic
Thanks in advance
Edited by madelingirly, 24 September 2015 - 06:48 PM.