Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

EGFR degradation


  • Please log in to reply
2 replies to this topic

#1 Niccolo

Niccolo

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 24 September 2015 - 05:58 PM

When added EGF 1nM to cancer cell lines (nasopharyngeal carcinoma), and found decreased expression level of EGFR; does this mean EGFR degradation? why added EGF induced decline of EGFR expression? Thanks.



#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,221 posts
484
Excellent

Posted 29 September 2015 - 11:55 AM

How are you measuring the decrease? Is it possible that (if using western blotting) that the proteins are phosporylated after activation and this is blocking the antibody epitope?



#3 Niccolo

Niccolo

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 17 October 2015 - 08:01 AM

but is it correct that EGFRs are degraded only after activation/phosphorylation? Process of ligand-binding, dimerization, phosphorylation, ubiquitination, .... all the way to either at the end either recycled back to cell surface, or degradation?






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.