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Human cell membrane protein Western blot!

membrane protein western blot

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#1 Jeong-A

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Posted 21 September 2015 - 03:52 AM

Hi!!!!!!!!!!!!!!!

 

I am studying for a protein.

 

I want to have a Human Cell membrane protein electrophoresis.

 

I maked cell lysate, I want loading to SDS-PAGE using the pellets.

 

I use a buffer of Roche Company.

 

What should I do?

 

Do you know how to dissolve the pellets?

 

help~~~~ me~~~~~~

 

Please let me know if you have any other method.



#2 mdfenko

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Posted 22 September 2015 - 04:20 AM

you should be able to dissolve the pellet by heating with the sds-page loading buffer. just don't try to solubilize too much pellet in too little buffer.


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#3 Jeong-A

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Posted 23 September 2015 - 09:26 PM

you should be able to dissolve the pellet by heating with the sds-page loading buffer. just don't try to solubilize too much pellet in too little buffer.

Oh! Thank you.
 
Your answer has been a great help to me.






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