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Need to include protease inhibitor in IP wash buffer?

Immunoprecipitation Protease inhibitor Wash buffer

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#1 5280



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Posted 19 September 2015 - 07:30 PM

Is it necessary to include a protease inhibitor in the IP wash buffer? My lysates have protease inhibitors during incubation with antibody and beads but the wash is short and done at 4C and tubes kept on ice. Therefore I was not sure if the wash buffer needed the protease inhibitor. Seems kind of wasteful. 



#2 gvbdxz


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Posted 21 September 2015 - 03:30 AM

I always use protease inhibitors - lysis and washes. What would be wasteful is to have a sub-optimal experiment because of a change in protocol. At least that's how I see it.

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