Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Cleaning loading dye for PCR sequencing

PCR

  • Please log in to reply
No replies to this topic

#1 Raygoza

Raygoza

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 46 posts
0
Neutral

Posted 18 September 2015 - 08:13 AM

Hi, I sometimes use a polymerase with added loading green dye, has anyone been successful in cleaning it using ethanol precipitation or phenol:chloroform elution? 

 

I tried ethanol precipitation and the resulting elutant was still pretty green.

 

 

 Thanks







Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.