Hi, I sometimes use a polymerase with added loading green dye, has anyone been successful in cleaning it using ethanol precipitation or phenol:chloroform elution?
I tried ethanol precipitation and the resulting elutant was still pretty green.
Thanks
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Andrea Fortina
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Posted 21 June 2016 - 05:47 PM
I think it is impossible to clean the green dye by that way, you'd better use new polymerase.
Andrea Fortina
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CreativeBiomart, Recombinant protein expert
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