Hi, I sometimes use a polymerase with added loading green dye, has anyone been successful in cleaning it using ethanol precipitation or phenol:chloroform elution?
I tried ethanol precipitation and the resulting elutant was still pretty green.
Thanks
Submit your paper to J Biol Methods today!
1 reply to this topic
#2
Andrea Fortina
-
- Active Members
-
- 55 posts
Enthusiast
1
Neutral
Neutral
Posted 21 June 2016 - 05:47 PM
I think it is impossible to clean the green dye by that way, you'd better use new polymerase.
Andrea Fortina
Genius only means hard-working for all one's life.
CreativeBiomart, Recombinant protein expert
Also tagged with one or more of these keywords: PCR
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
Problematic DNA ExtractionStarted by hmwmx, 14 Apr 2017  extraction, pcr, dna
|
|
|
||
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
Recombinant DNA vaccine productionStarted by Mashudu, 02 Feb 2017 Primer design, Molecular biology and 3 more...
|
|
|
||
Protocols and Techniques Forums →
siRNA, microRNA and RNAi →
Where can I find 100-200 unique primer sequences for preparation of arrayStarted by Bio21, 16 Jan 2017 shRNA, array, PCR, primer
|
|
|
||
Products and Vendors Discussion →
PCR Reagents and Equipments →
PCR cycler with end product fluorescence detectorStarted by Indrek Keskkyla, 05 Jan 2017 cycler, PCR and 1 more...
|
|
|
||
Protocols and Techniques Forums →
Molecular Biology →
Site directed mutagenesis stopped working.Started by Akash Deep, 25 Oct 2016 PCR, SIte directed mutagenes
|
|
|
