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Cleaning loading dye for PCR sequencing

PCR

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#1 Raygoza

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Posted 18 September 2015 - 08:13 AM

Hi, I sometimes use a polymerase with added loading green dye, has anyone been successful in cleaning it using ethanol precipitation or phenol:chloroform elution? 

 

I tried ethanol precipitation and the resulting elutant was still pretty green.

 

 

 Thanks



#2 Andrea Fortina

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Posted 21 June 2016 - 05:47 PM

I think it is impossible to clean the green dye by that way, you'd better use new polymerase. 


Andrea Fortina

Genius only means hard-working for all one's life.

CreativeBiomart, Recombinant protein expert

 

 






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