So I typically run western blots with no problem. However, during my most recent experiment something happened which I can't figure out the reason for.
So I loaded my samples into my gel like always. The gel seemed fine and everything went as usual. I ran the gel at 120V for 2 hours. However, upon removing the gels I saw that both of the gels were full of bubbles which didn't exist before running the gel.
I'd appreciate it if someone could let me know why this happened and how to prevent this from happening again.
Thanks a lot for your help.