Now, the question title may sound weird right off the bat, but my question is as follows:
Some restriction enzymes warn about the DNA mobility changing properties of some enzymes because the enzyme may remain attached to the DNA, so my question is can i add SDS to the loading dye and perhaps resolve this problem to detach proteins off the DNA ? i wanted to make sure that SDS doesn't have DNA denaturing properties as well. or if the idea sounds reasonable.
I know that you may inactivate some of the enzymes before electrophoresis but please focus on my question as it is.
Edited by Raygoza, 07 September 2015 - 04:49 AM.