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lysate protein concentration


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#1 Raygoza

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Posted 06 September 2015 - 08:25 AM

Is it possible to concentrate the bacterial lysate for functional assays (no western blots)? is the speedvac ok?

 

Thanks


Edited by Raygoza, 06 September 2015 - 08:25 AM.


#2 labtastic

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Posted 06 September 2015 - 03:45 PM

Speed vac is not recommended for concentrating protein for functional assays. It'll increase the concentration of everything (salts, nucleic acids, etc) and will warm up your sample.

 

Instead, use a small filter concentrator. You can buy 0.5ml centrifuge concentrators that will concentrate your protein but not the salts, etc. 

 

http://www.emdmillip...on,MM_NF-C82301


Edited by labtastic, 06 September 2015 - 03:45 PM.





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