Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Confused with the CALCULATION statement

calculation ELISA Molecular biology

  • Please log in to reply
1 reply to this topic

#1 Mad Researcher

Mad Researcher

    Newbie !!

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 205 posts
12
Good

Posted 06 September 2015 - 02:16 AM

Hello,

 

I am getting confused with the Calculation statement given in the ELISA protocol.

 

This is for preparing reagents and it says that:

 

"Dilute 50 ml of Wash Buffer Concentrate into de-ionized or distilled water (dH2O) to a final volume of 500ml". Does this mean I take 50 ml of wash buffer + 450 mL of water

 

OR

 

Does this mean I do 50*x = 1*500

x = 500/50 = 10 ml of wash buffer + 490 ml of water

 

Kindly advice.


Cheers,

Mad Researcher

#2 phage434

phage434

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 2,733 posts
298
Excellent

Posted 06 September 2015 - 04:57 AM

It means your first option.  Frankly, I can't imagine how you think it could mean the second.







Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.