I am getting confused with the Calculation statement given in the ELISA protocol.
This is for preparing reagents and it says that:
"Dilute 50 ml of Wash Buffer Concentrate into de-ionized or distilled water (dH2O) to a final volume of 500ml". Does this mean I take 50 ml of wash buffer + 450 mL of water
Does this mean I do 50*x = 1*500
x = 500/50 = 10 ml of wash buffer + 490 ml of water