I often make sterile 40% glucose , 1 liter, by just autoclaving it.
After autoclaving I use this glucose to add to the medium.
I just store it at roomtemperature.
And no: it does not turn brown.
After autoclaving, its best to just shake the bottle a bit, to make sure its mixed well.
I Always do it like this and never had problems with it. Many others in the lab do it like this as well.
if you do make it: use a big magnet to stirr it when you are preparing it because you really need to add a lot of glucosepowder. I Always put some water (lets say 200ml) in my bottle, with a magnet, and then I add the glucose, 100gram per time.
And I add some more water during the preparation untill I have a total volume of 1 liter (glucose+water).
Pito, I was warned not to autoclave glucose (with media).
So you mean if autoclaving glucose alone is not a problem?
Would the solution turn brown (burned) like how when we heated sugar?
Also, do we store the glucose solution in 4 degree or room temperature?