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Western Blot after Coomassie stain of antibody?


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#1 Elobash

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Posted 10 August 2004 - 06:40 AM

Is it possible to do Western Blotting after Coomassie staining the mb?
Cheers

#2 Dicky Mac

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Posted 26 August 2004 - 07:58 AM

I assume you mean Coumassie the gel...

If so, I've used Invitrogen's simplyblue (a great stain). It comes straight out of the gel in the methanol used for western transfer (typically 10-20%)

Does this help?

Dicky




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