Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

pIKK antibody for western blot (RAW 264.7 cells) - any recommendations?

pIKK RAW264.7 western blot antibodies

  • Please log in to reply
1 reply to this topic

#1 murusa

murusa

    member

  • Active Members
  • Pip
  • 15 posts
0
Neutral

Posted 30 August 2015 - 05:25 PM

Hi everyone!

Can anyone recommend a good pIKK antibody for western blot? we have checked pIKK in RAW 264.7 cells and it was ok with an old stock of antibodies, but then we ordered the new one vial (seems to be same catalog number), but result looks so bad that we cannot see any difference between samples, background is very high. We tried different ways to reduce background but still no result: the signal is very low and background is too high. I feel there is a problem with the stock so I would like to order another one, maybe different vendor.

we have been using pIKKalpha/beta from Cell Signaling cat #2697L

 

If you tried any other antibodies and got good results please let me know the catalog number, we will try it!

 

thank you in advance!



#2 GonzaloD_Biomed

GonzaloD_Biomed

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 19 May 2016 - 12:56 AM

Hi, from your description I understand you want to study the same phosphorylations in IKKα/β, that is Ser176 and 180. By searching in Labome's VAD (validated antibody database), and filtering for "mouse" reactivity and "western blot" application, you can see all antibodies used in recent publications:

 

http://www.labome.co...rs=10090&appl=1

 

There are two other antibodies that can be good alternatives: one from Bioss (cited once) and one from Abcam (cited 5 times).

 

http://www.labome.co...s/bs-3237R.html

 

http://www.labome.co...am/ab17943.html

 

I hope this helps.







Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.