I am trying to extract RNA from embryonic mouse hearts. I have a few questions, what are people's methods of disruption/homogenization-we don't have any tools for that right now, I am wondering if there is anything cheap I can buy. Also, while I do the dissections, is it possible to put the tissue immediately in the RLT that comes with the Qiagen kit? I tried putting it in RNA later, but when I tried to homogenize with a tube mortar and pestle, I couldn't grind the tissue because it got so hard in the RNA later solution. And finally, do people find that the trizol method is better than the RNA kits from qiagen, and if so does anyone have a protocol?