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downregulation of protein with ON-TARGET plus Non-targeting pool - Dharmacon

siRNA Dharmacon off target deletion cell lines

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#1 Madhura Panse

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Posted 19 August 2015 - 01:53 AM

Hello everybody,

 

Has anybody faced this problem before while working with cell lines:

 

downregulation/degradation of protein with 'ON-TARGET plus Non-targeting pool' (control siRNA) product from Dharmacon? The product number is: D-001810-10-05.

 

Thanks!

 

Madhura



#2 GNANA

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Posted 19 August 2015 - 10:42 AM

I think you seeing this because of toxicity.  you might  have to titrate down the Dharmafect and the siRNA concentration.


I always had an alternate hypothesis....






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