I did a Western with several lysates of different cell lines, always treated with DMSO or a drug (always the same).
After Ponceau staining, I see that some cell lines did not get transferred - either the high molecular proteins or even any bands. I treated all cell pellets the same way - used RIPA buffer to lyse them and added the same sample buffer, so I really don't have a clue what happened...? It does not look like bubbles or anything because it is very restricted to the two lanes of the same cell lines.
Thanks a lot for any advice!