Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Migration of circular DNA vs circular/crosslinked peptide in gels


  • Please log in to reply
2 replies to this topic

#1 labtastic

labtastic

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 179 posts
16
Good

Posted 11 August 2015 - 12:54 PM

As I understand it:

 

1. Relaxed circular DNA migrates slower on a gel than linearized DNA (e.g. here)

 

2. Circular peptides (or intra-crosslinked peptides) run faster on a gel than linearized peptide

 

--> So circular DNA runs slower and circular peptides run faster than their linear counterparts.

 

 

Why the difference? 


Edited by labtastic, 11 August 2015 - 05:33 PM.


#2 Andrea Fortina

Andrea Fortina

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 55 posts
1
Neutral

Posted 21 June 2016 - 06:39 PM

I do not see any relationship between DNA and peptides from your condition, how did you get such conclusion?


Andrea Fortina

Genius only means hard-working for all one's life.

CreativeBiomart, Recombinant protein expert

 

 


#3 labtastic

labtastic

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 179 posts
16
Good

Posted 24 June 2016 - 11:40 AM

Circular, or internally crosslinked peptides run faster on an acrylamide (sieving) gel than its linearized counterpart. I have seen this many times and is pretty common knowledge. This makes sense because circular (e.g. disulfide intra-chain crosslinked) peptides are more compact than their linear (e.g. fully reduced) counterparts, and thus travel through the acrylamide matrix faster.

 

On the other hand, relaxed circular (e.g. nicked non-supercoiled) DNA travels slower than than its linearized (e.g. digested) counterpart in an agarose (sieving) gel. For example, see this gel

 

Same principles, same concept, yet two different migration patterns. 






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.