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Glycoproteins on SDS-PAGE

Started by cellbiol, Aug 10 2004 02:03 AM

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#1 cellbiol

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Posted 10 August 2004 - 02:03 AM

Hi Everybody

Can anybody help me on how to selectively stain glycoproteins on SDS-PAGE gel or on PVDF/Nylon membrane??

Thanx
Cell biol

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#2 eric1turgeon

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Posted 10 August 2004 - 12:11 PM

Hi!

You might want to try lectins, wich usually bind specifically to glycoproteins.
Here's a link you might find interesting... http://www.probes.co...ables/0418.html
some nice figures...
Hope I could help!

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#3 wirly

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Posted 10 August 2004 - 03:17 PM

You may want to try this prodcut from Pierce: GelCode Glycoprotein Staining Kit

http://www.piercenet.com/Objects/View.cfm?...ly&ID=02051020#

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#4 laurence.ylao

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Posted 26 October 2009 - 07:45 AM

Hi, you may try the alcian blue staining with silver staining as below:

Combined alcian blue and silver staining of subnanogram quantities of proteoglycans and glycosaminoglycans in sodium dodecyl sulfate-polyacrylamide gels.

Møller HJ, Heinegård D, Poulsen JH.

Anal Biochem. 1993 Feb 15;209(1):169-75.

PMID: 8465952

Good luck!

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