3 replies to this topic

[cellbiol]

Hi Everybody

Can anybody help me on how to selectively stain glycoproteins on SDS-PAGE gel or on PVDF/Nylon membrane??

Thanx
Cell biol

[eric1turgeon]

Hi!
You might want to try lectins, wich usually bind specifically to glycoproteins.
Here's a link you might find interesting... http://www.probes.co...ables/0418.html
some nice figures...
Hope I could help!

[wirly]

You may want to try this prodcut from Pierce: GelCode Glycoprotein Staining Kit

http://www.piercenet...ly&ID=02051020#

[laurence.ylao]

Hi, you may try the alcian blue staining with silver staining as below:

Combined alcian blue and silver staining of subnanogram quantities of proteoglycans and glycosaminoglycans in sodium dodecyl sulfate-polyacrylamide gels.

Møller HJ, Heinegård D, Poulsen JH.

Anal Biochem. 1993 Feb 15;209(1):169-75.

PMID: 8465952

Good luck!