I am trying to figure out the best way to store my ligation mixture before I perform a transfection.
I am hoping to clone my insert into pCDNA3.
My question is can I first ligate my insert into pCDNA3, transform into bacteria and make glycerol stocks with these colonies before I do the transfection?
Originally I was thinking of immediately transfecting after I performed the ligation. It seems like it would be easier though to prepare the DNA from the glycerol stock.