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Good parafilm


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18 replies to this topic

#1 pito

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Posted 04 August 2015 - 08:20 AM

Hello all,

 

I am looking for a good brand of parafilm.

At the moment I am using parafilm from "parafilm" itself (parafilm M), but this one starts to melt even at 37°C after 1 day of incubation.

Not acceptable for me.

 

So any other brands you are familiar with?

 

Other also had this problem of melting parafilm at even low (37°C) temperatures?

 

 

 


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#2 phage434

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Posted 04 August 2015 - 10:48 AM

Probably not going to solve your problem, but I use plastic bags instead of parafilm on my plates. Easy, quick, and they don't melt.



#3 pito

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Posted 04 August 2015 - 11:36 AM

I was thinking the same, but wonder if it would really help.

I have to incubate over a period of 10-15 days at 37°C and using the bag alone, I wonder if this would do the trick.

You close the bag by taping it shut or?


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#4 bob1

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Posted 04 August 2015 - 12:27 PM

You close the bag by taping it shut or?

I use a heat sealer to shut the bag , creates a perfect seal. Another good option is to use zip-lock bags or get some bag snap seals (don't know the proper term, but basically a crocodile clip that can be used to close the bag).



#5 hobglobin

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Posted 04 August 2015 - 01:02 PM

Usually Parafilm should melt at 60°C, therefore I'd complain.
Nescofilm was an alternative, but seems to be cancelled (Fisher Scientific).



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#6 pito

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Posted 05 August 2015 - 08:03 AM

Usually Parafilm should melt at 60°C, therefore I'd complain.
Nescofilm was an alternative, but seems to be cancelled (Fisher Scientific).

I know!

I complained, but complaining is not solving my problem haha.


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#7 pito

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Posted 05 August 2015 - 08:04 AM

 

You close the bag by taping it shut or?

I use a heat sealer to shut the bag , creates a perfect seal. Another good option is to use zip-lock bags or get some bag snap seals (don't know the proper term, but basically a crocodile clip that can be used to close the bag).

 

 

I was thinking something similar, but I wonder if this would do the trick for an incubation period of 10-15 days....


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#8 hobglobin

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Posted 05 August 2015 - 09:38 AM

Not really sure for what you use it, needs it to be permeable to air? I guess then you have no or very few alternatives...

Aluminium or plastic wrap might be alternatives too, though you cannot look them completely.

If you need a bag for incubation of Petri dish piles, we use the original plastic bag and seal it with a locking clip or a peg.



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#9 phage434

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Posted 05 August 2015 - 09:57 AM

The major problem at 10-15 days in a bag tends to be mold growth. But you may be more sterile than I am.



#10 pito

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Posted 05 August 2015 - 10:11 AM

Not really sure for what you use it, needs it to be permeable to air? I guess then you have no or very few alternatives...

Aluminium or plastic wrap might be alternatives too, though you cannot look them completely.

If you need a bag for incubation of Petri dish piles, we use the original plastic bag and seal it with a locking clip or a peg.

well, I am doing some yeast two hybrid screenings.

So permeable to air would be something I want/need.


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#11 pito

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Posted 05 August 2015 - 10:12 AM

The major problem at 10-15 days in a bag tends to be mold growth. But you may be more sterile than I am.

That is my worry as well, especially since we do have lots of fungal spores flying around.


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#12 jamieoverton727

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Posted 13 August 2015 - 09:26 AM

The Parafilm M sealing film have become so successful that new products have been introduced for specific markets such as Parafilm Budding (Grafting) Tape, Parafilm Florist Stem Wrap, and Floratape. A comparison between these different Parafilm products can be understood in a very short time period.

 

copied from: http://www.2spi.com/...pp/parafilm.php


Edited by hobglobin, 17 August 2015 - 01:41 PM.
comment to add source


#13 Trof

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Posted 17 August 2015 - 11:37 AM

I'm not sure about that but.. Saran wrap? That should not melt in 37.  But I don't know, we don't parafilm petri dishes at all. Not in the incubator anyway, just for the fridge storage afterwards.

And if you need a petri dish that is permeable by air (I though that parafilm would seal it airtight though).. and at the same time not get a mold inside.. seems problematic.


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#14 El Crazy Xabi

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Posted 17 August 2015 - 07:19 PM

Parafilm is permeable to gases, but, off course, the gas exchange won't be the same as in unsealed plates

 

http://www.parafilm....ucts#parafilm-m



#15 pito

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Posted 18 August 2015 - 12:16 AM

Parafilm is permeable to gases, but, off course, the gas exchange won't be the same as in unsealed plates

 

http://www.parafilm....ucts#parafilm-m

thats the one we use/have, but its not good...
 


If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.





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