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[anonymous]

I think you can find the protocol you need under the category DNA>Oligos. there are several protocols for this purpose. I myself have tried those protocols. They worked fine.The following is the protocol I used to prepare oligos for bandshift assay

  
1. Purify    ds oligos with ss oligos by polyacrylamide gel electrophoresis  
       
   1. Prepare      by polyacrylamide gel  
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        i.  30% polyacrylamide (37.5:1)      10ml

         
         
         
         
      ii.  1X TBE buffer     
         
       40ml

         
         
         
        iii.  TEMED    
         
         
        40ul

         
         
         
       iv.  APS    
         
         
        400ul

        
          
      2. Add      1X loading dye and load all of the sample to the wells and run gel at 150V    
      3. Stain      gel for 30’ in ethidium bromide.( or in a dark room, place a fluorescent      thin-layer chromatographic plate under the gel and examine the gel by      illumination from above using a hand-held, long-wavelength UV lamp. The      DNA absorbs the UV light and appear dark bands against a uniform      fluorescent background)  
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      4. Cut    band and crush the gel into a slurry. Transfer the slurry into 3 or 4 tubes.    Add 1ml  elution buffer (0.1%    SDS, 0.5M Ammonium acetate, 10mM Magensium acetate) incubate at 37C with    shaking overnight.  
      5. Centrifuge    the tubes at 12000g for 5min. transfer the pooled supernatants to a 5ml    syringe and pass them through a filter (Millipore, 0.45-micron pore size).    Collect the effluent in a 15ml tube. ( or filter the gel mixture through a    Amicon gel extraction column)  
      6. Add    2 vol 100% ethanol, and set at –70C for 30min, centrifuge for 20min and    wash with 75% ethanol. Resuspend in TE buffer.
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         If you need any further details, Please email me.