Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

very high salt concentration ??

gel electrophoresis tank

  • Please log in to reply
4 replies to this topic

#1 nightingale

nightingale

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 212 posts
10
Good

Posted 03 August 2015 - 01:23 AM

Dear All, 

 

kindly find the attached pictures of the gel electrophoresis tank used in my lab.

do you kindly see , where the salt is ?? and there is something yellowish too ... right ? 

 

this tank is used for gel ectrophoresis with 1X TBE buffer.

 

i dont know why i always end up with this high salt concentration..

 

is this normal ?? 

 

thank you smile.png

Attached Thumbnails

  • IMG_98631.JPG
  • IMG_98641.JPG
  • IMG_98651.JPG
  • IMG_98661.JPG
  • IMG_98671.JPG
  • IMG_98681.JPG
  • IMG_98691.JPG

" The more you learn, the more you realize how little you know ... "

#2 phage434

phage434

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 2,747 posts
304
Excellent

Posted 03 August 2015 - 04:52 AM

This is common and not a problem. It comes from buffer evaporating, leaving behind the buffer salts.  If it bothers you, wash it off with water.

T



#3 mdfenko

mdfenko

    an elder emeritus

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 3,369 posts
217
Excellent

Posted 03 August 2015 - 04:55 AM

you will have migration of the buffer salts to the electrodes.

 

it looks to me that the apparatus is not cleaned regularly.

 

do you leave the buffer in the apparatus between uses? (as phage434 wrote as i was) evaporation of the buffer will leave residue behind.

 

the yellow appears to be due to corrosion.


talent does what it can
genius does what it must
i used to do what i got paid to do


#4 nightingale

nightingale

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 212 posts
10
Good

Posted 03 August 2015 - 04:55 AM

thanks a bunch phage434 :)


" The more you learn, the more you realize how little you know ... "

#5 nightingale

nightingale

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 212 posts
10
Good

Posted 03 August 2015 - 04:59 AM

yes medfenko , we leave the buffer inside the tank between the "usage times".

 

i took the pictures today, while the tank was last washed properly maybe one month ago. 


" The more you learn, the more you realize how little you know ... "




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.