Method to look for novel methylated gene
Posted 09 August 2004 - 10:31 PM
Posted 10 August 2004 - 12:39 AM
1. CpG island microarray
2. Gene expression microarry approach: treat your cells with demethylation agents such as azo-cytidine, find genes whos expression is upregulated by treatment as your candidate gene for methylation mapping
4. If you can find existing expression data such as EST, SAGE, microarray in NCBI's database for NPC, genes that are expressed lower in cancer may be good candidates.
5. If you have some candidate genes in mind such as 10-20, just screen them one by one.
Posted 10 August 2004 - 08:54 PM
In your reply, you mentioned about CpG islands microarray. is the microarray commercially available or i have to constrcut myself? what genes are spotted on the microarray? is it cancer specific CpG islands?
Posted 10 August 2004 - 09:09 PM
Yes there are commercial (not really) cpg microarray available at
Look for "Human CpG 12K Arrays"