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Solubilizing agarose in soft agar assay for absorbance reading

agarose soft agar assay solubilize anchorage independent cell culture

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#1 TinaTeaspoon

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Posted 29 July 2015 - 09:39 AM

Does anybody have an in house buffer/protocol for solubilizing the agarose/ agar in a soft agar assay?

 

 

I wish to stain my colonies (with haematoxilin or crytal violet) and then solubilize the whole thing and read the absorbance on a plate reader.

 

I know there are certain kits that inadvertantly solubilze the agarose gel (e.g. CellGlo 2.0 from Promega, but I dont have access to a luminometer). So it can be done.

 

Thanks in adance



#2 bob1

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Posted 29 July 2015 - 12:58 PM

You could try using an agarase and some gentle heat.







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