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Solubilizing agarose in soft agar assay for absorbance reading

agarose soft agar assay solubilize anchorage independent cell culture

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#1 TinaTeaspoon



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Posted 29 July 2015 - 09:39 AM

Does anybody have an in house buffer/protocol for solubilizing the agarose/ agar in a soft agar assay?



I wish to stain my colonies (with haematoxilin or crytal violet) and then solubilize the whole thing and read the absorbance on a plate reader.


I know there are certain kits that inadvertantly solubilze the agarose gel (e.g. CellGlo 2.0 from Promega, but I dont have access to a luminometer). So it can be done.


Thanks in adance

#2 bob1


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Posted 29 July 2015 - 12:58 PM

You could try using an agarase and some gentle heat.

Also tagged with one or more of these keywords: agarose, soft agar assay, solubilize, anchorage independent, cell culture

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